Microthermal Techniques for Mixing, Concentration, and Harvesting of Dna and Other Microdroplet Suspensions
نویسندگان
چکیده
The use of microprobes to manipulate, concentrate, and sample aqueous droplets within an oil phase on a blank substrate is explored. In particular, this paper introduces a number of thermal-based techniques which make use of a point heat source to perform high speed mixing of droplets at speeds up to 300 rpm, collection and merging of droplets, concentration of suspended particles through controlled evaporation of the droplet, and precipitation of low concentration suspensions such as DNA onto a microprobe tip. Quantities of DNA as low as 10 ng have been sampled on a 15 μm diameter tip.
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